Services and Rates

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Overview

At the UC Riverside Metabolomics Core, we have several LC-MS and GC-MS platforms geared towards specific classes of metabolites. For more comprehensive coverage, a single sample or metabolite extract can be analyzed on several different platforms. 

Sample cost is largely dependent on sample preparation complexity and sample set size.  Please contact the Metabolomics Core Team (metabolomics@ucr.edu) to discuss options and pricing for your project.

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All services include:
Sample preparation
(lyophilization, homogenization)
Metabolite extraction
(monophasic, biphasic, solid-phase extraction)
Data collection
(UPLC metabolite separation coupled to MS detection)
Data processing 
(peak finding, alignment, integration, normalization, metabolite ID)
Statistical analysis with interactive plots
(univariate, multivariate, post-hoc analysis, heat maps, PCA plots, box plots, violin plots, volcano plots)


Central Carbon Metabolism 

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Over 100 constituents of central carbon metabolism are typically
detected with this approach:
Sugar phosphates (Glycolysis)
Organic acids (TCA cycle)
Amino acids
Purines
Pyrimidines


Lipidomics

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Up to 300 lipids can be detected with this approach:
Sphingomyelins
Ceramides
Phospholipids
Sterols
Fatty acids
Triglycerides


Secondary Metabolites

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The number and type of secondary metabolites detected with this approach is largely dependent on sample type, but typically include:
Flavonoids
Glycosides
Anthocyanins
Sterols
Phenolics
Alkaloids


Neutral Sugars

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A GC-MS assay capable of separating several neutral sugar isomers:
Monosaccahrides
Disaccharides
Trisaccharides


Phytohormones

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A panel of 8 phytohormones:
Auxins (IAA, ICA)
Cytokinins (trans-Zeatin)
Jasmonic acid
Jasmonic acid-Isoleucine
OPDA
Abscisic acid
Salicylic acid


Phytohormones Example Data

8 phytohormones can be detected in a single injection. The data below were collected on leaf samples on the UC Riverside campus. Leaves were wounded, or not, for 3 hours. Leaves were then quickly harvested and snap frozen in liquid nitrogen. After freeze drying, leaf material was homogenized to a fine powder, then 10 mg was weighed and phytohormones were extracted then quantified by LC-MS. The method can be applied to various species and sample types (e.g. roots, shoots, and leaves). The number of biological replicates is dependent on experimental conditions, though at least n=4 is recommended. Contact Metabolomics Core Team (metabolomics@ucr.edu) to discuss your project or for a cost estimate.  Method Reference

Phytohormones analysis

 

 
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