Mass Spectrometry
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Waters Synapt G2-Si Q-TOF mass spectrometer with ion mobility coupled to a Waters I-class UPLC. Used for untargeted metabolomics analysis including secondary metabolites and lipidomics as well as targeted metabolite analysis where high-resolution MS is needed. |
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Waters MALDI Imaging source coupled to the Synapt G2-Si Q-TOF with ion mobility. Used for MS imaging experiments and as a MALDI-TOF instrument. Tissue imaging experiments can be conducted with resolution as low as 25 microns. |
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Waters G2-XS Q-TOF mass spectrometer coupled to a Waters H-class UPLC with a fractionation manager. Used for untargeted metabolomics and lipidomics analysis, as well as targeted metabolite analysis where high-resolution MS is needed. The cooled fractionation manager allows for the collection of up to 96 fractions from a single sample, enabling both targeted metabolite fractionation and a high-throughput workflow for activity-guided fraction characterization. |
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Waters TQ-XS triple quadrupole mass spectrometer coupled to a Waters I-class UPLC. Used for targeted metabolomics and lipidomics analyses including central carbon metabolism, oxylipins, and phytohormones. |
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Thermo Scientific ISQ 7000 GC-MS system with liquid and headspace technology including Solid Phase Micro Extraction (SPME). The system is equiped with the NIST mass spectral library that identifies metabolites by providing reference mass spectra. Used for the analysis of a wide variety of metabolites. |
Sample Preparation
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Lab Conco Lyophilizer (freeze dryer), (removal of water from tissue or samples) |
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Omni bead ruptor homogenizer with cryo control unit for 2,7, and 30 ml grinding tubes (tissue homogenization) |
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Vortex mixer and Ultrasonic water bath (metabolite extraction, cell disruption) |
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Thermo Fisher Refrigerated Benchtop Centrifuge with rotors for 1.5 ml tubes, 2ml glass vials, and plates (pellet protein and insoluble material) |
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Nitrogen evaporator for 48 position with digital temperature control (concentrate metabolite extracts or fractions) |
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Air brush pen for matrix application on sample sections (imaging experiments) |