Overview
At the UC Riverside Metabolomics Core, we have several LC-MS and GC-MS platforms geared towards specific classes of metabolites. For more comprehensive coverage, a single sample or metabolite extract can be analyzed on several different platforms. 

Most services start at around $74/sample, but cost is largely dependent on sample preparation complexity and sample set size. Please contact the Metabolomics Core lead Jay Kirkwood (jkirk@ucr.edu) to discuss options and pricing for your project.

 

 

All services include:
Sample preparation
(lyophilization, homogenization)
Metabolite extraction
(monophasic, biphasic, solid phase extraction)
Data collection
(UPLC metabolite separation coupled to MS detection)
Data processing 
(peak finding, alignment, integration, normalization, metabolite ID)
Statistical analysis with interactive plots
(univariate, multivariate, heat maps, PCA plots, box plots, volcano plots)

 

 


Central Carbon Metabolism
Over 100 constituents of central carbon metabolism are typically
detected with this approach:
Sugar phosphates (Glycolysis)
Organic acids (TCA cycle)
Amino acids
Purines
Pyrimidines


Lipidomics
Up to 300 lipids can be detected with this approach:

Sphingomyelins
Ceramides
Phospholipids
Sterols
Fatty acids
Triglycerides


Secondary Metabolites
The number and type of secondary metabolites detected with this
approach is largely dependent on sample type, but typically include:
Flavonoids
Glycosides
Anthocyanins
Sterols
Phenolics
Alkaloids


Neutral Sugars
A GC-MS assay capable of separating several neutral sugar isomers:
Monosaccahrides
Disaccharides
Trisaccharides


Phytohormones
A panel of 8 phytohormones (more info):

Auxins (IAA, ICA)
Cytokinins (trans-Zeatin)
Jasmonic acid
Jasmonic acid-Isoleucine
OPDA
Abscisic acid
Salicylic acid