Facility Description for Grant

Mass Spectrometry


Waters Synapt G2-Si Q-TOF mass spectrometer with ion mobility coupled to a Waters I-class UPLC. Used for untargeted metabolomics analysis including secondary metabolites and lipidomics. With collision cells before and after the mobility cell, this instrument is also useful for the characterization of unknowns, as time assisted parallel fragmentation can be performed to generate pseudo MS3 spectra.

 

 

 

 

 

 


Waters G2-XS Q-TOF mass spectrometer coupled to a Waters H-class UPLC with a fractionation manager. Used for untargeted metabolomics and lipidomics analysis, as well as targeted metabolite analysis where high resolution MS is needed. The cooled fractionation manager allows for collection of up to 96 fractions from a single sample, enabling both targeted metabolite fractionation and a high-throughput workflow for activity guided fraction characterization.

 

 

 

 



Waters TQ-XS triple quadrupole
 mass spectrometer coupled to a Waters two-dimensional I-class UPLC
. Used for targeted metabolomics and lipidomics analyses including central carbon metabolism, oxylipins, and phytohormones. The 2D UPLC allows for at-column dilutions of metabolite extracts and online SPE, improving sensitivity for many low level metabolites.

 

 

 

 

 

 

 

Sample Preparation

Freeze dryer with trays for bulk drying (removal of water from tissue for homogenization, concentrate water samples)

Cooled bead mill homogenizer, 7 mL or 1.5 mL tubes (tissue homogenization)

Ultrasonic water bath (metabolite extraction, cell wall disruption)

Centrifuge, rotors for 1.5 mL tubes and plates (pellet protein and insoluble material)

48-position heated nitrogen evaporator (concentrate metabolite extracts or fractions)