Metabolites represent one of the most diverse set of biomolecules varying greatly in abundance (> 6 orders of magnitude) and physicochemical properties (hydrophilic and hydrophobic). For this reason, no single analytical platform can analyze metabolites on a comprehensive level. However, as metabolome coverage increases, so does cost. At the UC Riverside Metabolomics Core Facility, we offer several assays that differ in targets of interest and breadth of metabolome coverage.

Assays can be combined in streamlined workflows to increase metabolome coverage. Costs associated with all assays and workflows include sample preparation, data collection and processing, and statistical analysis. 

 

 

Untargeted methods
The goal of untargeted metabolomics is to measure metabolites on a comprehensive level. The standard analysis yields a peak list that includes relative quantitation for metabolites identified by in-house library matching and unidentified, putative metabolites. Though the number of identified metabolites is improving, the majority of detected peaks are classified as unknowns.

 

INCLUDED IN UNTARGETED METABOLOMICS METHODS

  • Sample preparation (homogenization and metabolite extraction)
  • Analysis by LC-MS (LC separation and Q-TOF MS detection)
  • Quality control monitoring (system stability and accuracy)
  • Data processing (raw data to feature list, search against in-house metabolite library of > 3,000 compounds)
  • Statistical analysis and data visualization (univariate, multivariate, heatmap, correlation plot)
  • Storage of samples, metabolite extracts, and raw data
  • Data report and guidance (raw data, relative quantitation for identified metabolites and unidentified features, figures)

 

Semi-polar to non-polar metabolites
$42 – $75/sample*
The broadest metabolome coverage offered in a single LC-MS run. This method generally covers all metabolites more non-polar than phenylalanine:

    • Phenolics
    • Glycosides
    • Phospholipids
    • Anthocyanins
    • Neutral lipids
    • Sterols

Polar metabolites
$42 – $75/sample*
A solution for researchers interested in polar, primary metabolites:

    • Organic acids (TCA cycle)
    • Sugar phosphates (glycolysis, PPP)
    • Amino acids
    • Energy currency metabolites
    • Electron carriers
    • ROS metabolites

Lipids
$65 – $85/sample*
A solution for researchers interested in lipids. This method will focus on enhancing lipidome coverage and lipid identification. Currently under development.

 

 

Targeted methods
Targeted assays are generally less global than untargeted approaches, but with the benefit of increased sensitivity, these assays are geared towards low abundance metabolites.

 

INCLUDED IN TARGETED METHODS

  • Sample preparation (homogenization and metabolite extraction)
  • Analysis by LC-MS (LC separation and QQQ MS detection)
  • Quality control monitoring (system stability and accuracy)
  • Data processing (raw data to metabolite list, validation of peak integration)
  • Statistical analysis and data visualization (univariate, multivariate, heatmap, correlation plot)
  • Storage of samples, metabolite extracts, and raw data
  • Data report and guidance (raw data, relative quantitation for targeted metabolites, figures)

 

Polar metabolites
$55 – $85/sample*
This method is more focused than the untargeted approach, but with the benefit of increased sensitivity. Over 200 central carbon metabolites are targeted in this method:

    • Organic acids (TCA cycle)
    • Sugar phosphates (glycolysis, PPP)
    • Amino acids
    • Energy currency metabolites
    • Electron carriers
    • ROS metabolites

Oxylipins
Under development.

Phytohormones
Under development.

 

 

 

*Cost per sample is an estimate and may increase with complexity of sample preparation and small sample sets. Prices reflect UC system pricing. External academic rates are approximately 10% greater and commercial rates 30% greater. For an estimate of per sample cost for your specific project, please contact us.